High density cultivation of biomass in fed-batch system with DO-stat.

High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture

Microbial lipid production by the oleaginous yeast Rhorosporidium toruloides Y4 was studied using glucose as carbon source, in order to realize igh-density cell culture. Batch cultures demonstrated that there was little inhibitory effect with a substrate concentration of up to 150 g l−1. Flask ed-batch cultures were run for 25 days and reached a dry biomass and cellular lipid content of 151.5 g...

Fatty acid profile of Chlorella biomass obtained by fed batch heterotrophic cultivation

The cultivation of microalgae is of great interest due to the high yields and rapid growth rates that can be produced. The fatty acids from microalgal biomass may have therapeutic effects for humans and can be used for biodiesel production. The aim of this study was to evaluate the fatty acid profile of microalgae grown in a heterotrophic mode. Cultures that were carried out with BG11 medium su...

media and specific growth rate selection for high-cell-density cultivation of recombinant escherichia coli producing hGM-CSF in fed-batch process

Fed-batch cultivation of Saccharomyces cerevisiae in a hyperbaric bioreactor.

Fed-batch is the dominating mode of operation in high-cell-density cultures of Saccharomyces cerevisae in processes such as the production of baker's yeast and recombinant proteins, where the high oxygen demand of these cultures makes its supply an important and difficult task. The aim of this work was to study the use of hyperbaric air for oxygen mass transfer improvement on S. cerevisiae fed-...

Genetic Algorithms Based Parameter Identification of Yeast Fed-Batch Cultivation

Different kinds of genetic algorithms have been investigated for a parameter identification of a fermentation process. Altogether eight realizations of genetic algorithms have been presented four of simple genetic algorithms and four of multi-population ones. Each of them is characterized with a different sequence of implementation of main genetic operators, namely selection, crossover and muta...